Salmonella effector SopD promotes plasma membrane scission by inhibiting Rab10

Abstract Salmonella utilizes translocated virulence proteins (termed effectors) to promote host cell invasion. The effector SopD contributes invasion by promoting scission of the plasma membrane, generating -containing vacuoles. is expressed in all lineages and plays important roles animal models infection, but its targets are unknown. Here we show that can bind inhibit small GTPase Rab10, through a C-terminal activating protein (GAP) domain. During Rab10 effectors MICAL-L1 EHBP1 recruited sites. By inhibiting promotes removal recruitment Dynamin-2 drive membrane. Together, our study uncovers an role for regulating membrane identifies mechanism used bacterial pathogen manipulate this function during infection.